Only the job description changed.
| Previous Job Description | Current Job Description | ||||
|---|---|---|---|---|---|
| n | 1 | Job Description: | n | 1 | **This is a Part-Time, Limited Appointment position** |
| 2 | **This is a Part-Time, Limited Appointment position** This position is in the Department of Pediatri | ||||
| > | cs, Division of Pediatric Nephrology. The purpose of the position is to support research on the disc | ||||
| > | overy of small molecule modulators of ion transporters/channels important in human diseases, includi | ||||
| > | ng kidney diseases (kidney stones, kidney failure, hypertension), digestive disorders (diarrhea, con | ||||
| > | stipation, motility disorders), pulmonary diseases (asthma, cystic fibrosis), cardiovascular disease | ||||
| > | s (hypertension) and others. The Staff Research Associate (SRA) is expected to perform in vitro elec | ||||
| > | trophysiology in cells and tissues (Ussing Chamber), cell culture (maintaining and preparing cell li | ||||
| > | nes for experiments), measurement of ion transport in cells (via fluorescence using plate reader), p | ||||
| > | reparation of buffers and solutions for experiments, molecular biology (PCR, animal genotyping, West | ||||
| > | ern blot), staining techniques for tissues and cells (immunofluorescence, light microscopy etc.), an | ||||
| > | imal experiments (handling, anesthesia, injections, blood-urine-stool collections, colony maintenanc | ||||
| > | e, breeding, euthanasia, tissue harvesting, simple surgery), analysis of ions, biomarkers, chemical | ||||
| > | compounds and metabolites by ion chromatography and liquid chromatography-mass spectrometry. The SRA | ||||
| > | is going to perform the above mentioned work under supervision of the PI and will be trained by the | ||||
| > | PI for the above mentioned work. The SRA will have responsibility for animal safety and compliance, | ||||
| > | controlled substances and biological substances and will be responsible for protocol adherence and | ||||
| > | timely renewal and updating of the animal protocols in coordination with the PI. It is expected that | ||||
| > | the incumbent will work closely with IACUC, will determine what supplies are needed for experiments | ||||
| > | and organize their storage and use and will also assist with material transfers and safe shipping. | ||||
| > | Together with the other SRA’s in the lab, the incumbent is expected to perform shared general lab ma | ||||
| > | intenance duties such as defrosting freezers, cleaning hoods, incubators & sinks, organizing supplie | ||||
| > | s inventory, and overall lab maintenance & upkeep. The SRA will attend weekly laboratory meetings un | ||||
| > | less in direct time conflict with ongoing research/experiments, and will perform other duties as ass | ||||
| > | igned. | ||||
| 3 | 2 | ||||
| t | 4 | Qualifications: | t | 3 | This position is in the Department of Pediatrics, Division of Pediatric Nephrology. The purpose of |
| > | the position is to support research on the discovery of small molecule modulators of ion transporter | ||||
| > | s/channels important in human diseases, including kidney diseases (kidney stones, kidney failure, hy | ||||
| > | pertension), digestive disorders (diarrhea, constipation, motility disorders), pulmonary diseases (a | ||||
| > | sthma, cystic fibrosis), cardiovascular diseases (hypertension) and others. | ||||
| 5 | Required Qualifications: - BA/BS in a related science; or an equivalent combination of education and | 4 | |||
| > | experience - Experience with animal handling, genotyping, breeding, colony maintenance - Ability to | ||||
| > | work independently and to assess need for interaction with supervising researcher - Excellent oral | ||||
| > | and written communication skills, organizational and record keeping skills - Careful attention to de | ||||
| > | tail. Maintain a detailed laboratory notebook of experiments done on a daily basis Preferred Qualifi | ||||
| > | cations: - Experience with cell culture techniques including primary cell cultures, maintenance of c | ||||
| > | ell lines and transfection techniques - Coursework experience with standard molecular and biochemica | ||||
| > | l techniques including cell culture, PCR and western blotting - Masters of Science Degree, including | ||||
| > | one year experience | ||||
| 5 | The Staff Research Associate (SRA) is expected to perform in vitro electrophysiology in cells and t | ||||
| > | issues (Ussing Chamber), cell culture (maintaining and preparing cell lines for experiments), measur | ||||
| > | ement of ion transport in cells (via fluorescence using plate reader), preparation of buffers and so | ||||
| > | lutions for experiments, molecular biology (PCR, animal genotyping, Western blot), staining techniqu | ||||
| > | es for tissues and cells (immunofluorescence, light microscopy etc.), animal experiments (handling, | ||||
| > | anesthesia, injections, blood-urine-stool collections, colony maintenance, breeding, euthanasia, tis | ||||
| > | sue harvesting, simple surgery), analysis of ions, biomarkers, chemical compounds and metabolites by | ||||
| > | ion chromatography and liquid chromatography-mass spectrometry. The SRA is going to perform the abo | ||||
| > | ve mentioned work under supervision of the PI and will be trained by the PI for the above mentioned | ||||
| > | work. | ||||
| 6 | |||||
| 7 | The SRA will have responsibility for animal safety and compliance, controlled substances and biolog | ||||
| > | ical substances and will be responsible for protocol adherence and timely renewal and updating of th | ||||
| > | e animal protocols in coordination with the PI. It is expected that the incumbent will work closely | ||||
| > | with IACUC, will determine what supplies are needed for experiments and organize their storage and u | ||||
| > | se and will also assist with material transfers and safe shipping. Together with the other SRA’s in | ||||
| > | the lab, the incumbent is expected to perform shared general lab maintenance duties such as defrosti | ||||
| > | ng freezers, cleaning hoods, incubators & sinks, organizing supplies inventory, and overall lab main | ||||
| > | tenance & upkeep. The SRA will attend weekly laboratory meetings unless in direct time conflict with | ||||
| > | ongoing research/experiments, and will perform other duties as assigned. | ||||