Only the job description changed.
| Previous Job Description | Current Job Description | ||||
|---|---|---|---|---|---|
| n | 1 | Job Description: | n | 1 | The Huang Lab at UCSF (https://huanglabucsf.com/) investigates the genetics and biology of leukemia |
| > | through the use of relevant mouse and cell line models. Specific goals include: (1) to determine the | ||||
| > | biochemical mechanisms governing growth, survival and differentiation of leukemia cells; (2) to ide | ||||
| > | ntify genes and biochemical pathways that are regulated differentially in leukemia versus normal cel | ||||
| > | ls; (3) to measure the growth of distinct populations of normal and leukemia bone marrow cells in vi | ||||
| > | vo and in vitro; (4) to harvest murine bone marrow and embryonic fetal livers for transplantation in | ||||
| > | to irradiated recipients; (5) to transplant genetically modified hematopoietic cells into recipient | ||||
| > | mice; (6) to perform genetic analysis on mouse tissues; and, (7) to administer chemotherapeutic agen | ||||
| > | ts, radiation, and new experimental treatments to mice. | ||||
| 2 | The Huang Lab at UCSF ( https://huanglabucsf.com/ ) investigates the genetics and biology of leukemi | ||||
| > | a through the use of relevant mouse and cell line models. Specific goals include: (1) to determine t | ||||
| > | he biochemical mechanisms governing growth, survival and differentiation of leukemia cells; (2) to i | ||||
| > | dentify genes and biochemical pathways that are regulated differentially in leukemia versus normal c | ||||
| > | ells; (3) to measure the growth of distinct populations of normal and leukemia bone marrow cells in | ||||
| > | vivo and in vitro; (4) to harvest murine bone marrow and embryonic fetal livers for transplantation | ||||
| > | into irradiated recipients; (5) to transplant genetically modified hematopoietic cells into recipien | ||||
| > | t mice; (6) to perform genetic analysis on mouse tissues; and, (7) to administer chemotherapeutic ag | ||||
| > | ents, radiation, and new experimental treatments to mice. This Staff Research Associate (SRA) positi | ||||
| > | on is for an individual who will work on leukemia research in mouse models and cell lines. SRA dutie | ||||
| > | s will include: cell/tissue culture, biochemical assays (western blotting, immunoprecipitation, qPCR | ||||
| > | ), DNA and RNA extraction and manipulation, PCR and agarose gel electrophoresis, generation of recom | ||||
| > | binant retroviruses/lentiviruses and transduction of hematopoietic cells, flow cytometry, maintenanc | ||||
| > | e of accurate and complete records, organization and maintenance of mouse colonies (including comput | ||||
| > | erized record-keeping, breeding, monitoring and genotyping), and experimental procedures using labor | ||||
| > | atory mice (blood sampling, intravenous/intraperitoneal injection, dissection, analysis of harvested | ||||
| > | tissues). | ||||
| 3 | 2 | ||||
| t | 4 | Qualifications: | t | 3 | This Staff Research Associate (SRA) position is for an individual who will work on leukemia researc |
| > | h in mouse models and cell lines. SRA duties will include: cell/tissue culture, biochemical assays ( | ||||
| > | western blotting, immunoprecipitation, qPCR), DNA and RNA extraction and manipulation, PCR and agaro | ||||
| > | se gel electrophoresis, generation of recombinant retroviruses/lentiviruses and transduction of hema | ||||
| > | topoietic cells, flow cytometry, maintenance of accurate and complete records, organization and main | ||||
| > | tenance of mouse colonies (including computerized record-keeping, breeding, monitoring and genotypin | ||||
| > | g), and experimental procedures using laboratory mice (blood sampling, intravenous/intraperitoneal i | ||||
| > | njection, dissection, analysis of harvested tissues). | ||||
| 5 | Required Qualifications: - BA/BS in a related science; or an equivalent combination of education and | ||||
| > | experience Preferred Qualifications: - Expertise in breeding and maintaining laboratory mice - Mole | ||||
| > | cular biology skills including DNA/RNA extraction and PCR amplification - Tissue culture skills incl | ||||
| > | uding passaging cell lines - Biochemistry skills including Western blotting | ||||